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| Section | Weight | Objectives |
|---|---|---|
| Topic 1: Hematology | 20-25% | - Red blood cell physiology and disorders - Routine and automated hematology procedures - Hemostasis and coagulation testing - White blood cell morphology and diseases |
| Topic 2: Clinical Chemistry | 20-25% | - Electrolytes, acid-base balance, and blood gases - Endocrinology and therapeutic drug monitoring - Carbohydrates, lipids, proteins, and enzymes - Toxicology and liver/kidney function tests |
| Topic 3: Blood Banking (Immunohematology) | 15-20% | - Compatibility testing - Antibody detection and identification - Transfusion reactions and adverse events - Blood group systems and antigens - Blood components and storage |
| Topic 4: Laboratory Operations | 5-10% | - Pre-analytical, analytical, and post-analytical processes - Laboratory mathematics and instrumentation - Laboratory safety and regulatory compliance - Quality control and quality assurance |
| Topic 5: Microbiology | 15-20% | - Specimen collection and culture techniques - Identification of pathogenic microorganisms - Mycology, parasitology, and virology - Bacteriology and antimicrobial susceptibility |
| Topic 6: Urinalysis and Other Body Fluids | 5-10% | - Cerebrospinal, serous, and synovial fluids - Semen and amniotic fluid testing - Physical, chemical, and microscopic analysis - Urine formation and composition |
| Topic 7: Immunology and Serology | 5-10% | - Autoimmune and immunodeficiency disorders - Infectious disease serology - Immunologic testing methodologies - Immune system principles and components |
Using the formula on the right,
Cells/µL = 370 x 100 / 18 x 0.1
Cells/µL = 37000 / 1.8
Cells/µL = 20556 or 2.06 x 104
A sample of cerebrospinal fluid is diluted 1:100; the standard 9 squares of a hemocytometer are counted on each side for a total of 18 large squares.
Side 1-- 186 nucleated cells counted
Side 2-- 184 nucleated cells counted
total nucleated cells = 370
Using the standard hemocytometer formula shown on the right, what is the nucleated cell count per microliter (µL)?
Correct Answer: B 🗳️
The characteristic that distinguishes vancomycin-resistant Enterococcus faecium and Enterococcus faecalis from other Enterococcus species is their lack of motility.
Enterococci are all catalase-negative. Growth on bile esculin agar and in 6.5% salt broth are two characteristics that have commonly been used to identify Enterococcus to the genus level. A positive esculin in combination with a positive PYR reaction is another approach to presumptive identification.
Which one of these characteristics distinguishes vancomycin-resistant Enterococcus faecium and Enterococcus faecalis from other Enterococcus species?
Correct Answer: B 🗳️
The term universal precautions refers to:
Correct Answer: B 🗳️
Hematology
What is Coombs sera comprised of:
Correct Answer: C 🗳️
Which of these advancements in the clinical laboratory has led to concerns about patient privacy
Correct Answer: C 🗳️
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