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| Section | Weight | Objectives |
|---|---|---|
| Topic 1: Hematology | 20-25% | - Red blood cell physiology and disorders - Routine and automated hematology procedures - Hemostasis and coagulation testing - White blood cell morphology and diseases |
| Topic 2: Clinical Chemistry | 20-25% | - Electrolytes, acid-base balance, and blood gases - Endocrinology and therapeutic drug monitoring - Carbohydrates, lipids, proteins, and enzymes - Toxicology and liver/kidney function tests |
| Topic 3: Blood Banking (Immunohematology) | 15-20% | - Compatibility testing - Antibody detection and identification - Transfusion reactions and adverse events - Blood group systems and antigens - Blood components and storage |
| Topic 4: Laboratory Operations | 5-10% | - Pre-analytical, analytical, and post-analytical processes - Laboratory mathematics and instrumentation - Laboratory safety and regulatory compliance - Quality control and quality assurance |
| Topic 5: Microbiology | 15-20% | - Specimen collection and culture techniques - Identification of pathogenic microorganisms - Mycology, parasitology, and virology - Bacteriology and antimicrobial susceptibility |
| Topic 6: Urinalysis and Other Body Fluids | 5-10% | - Cerebrospinal, serous, and synovial fluids - Semen and amniotic fluid testing - Physical, chemical, and microscopic analysis - Urine formation and composition |
| Topic 7: Immunology and Serology | 5-10% | - Autoimmune and immunodeficiency disorders - Infectious disease serology - Immunologic testing methodologies - Immune system principles and components |
1. Using the formula on the right,
Cells/µL = 370 x 100 / 18 x 0.1
Cells/µL = 37000 / 1.8
Cells/µL = 20556 or 2.06 x 104
A sample of cerebrospinal fluid is diluted 1:100; the standard 9 squares of a hemocytometer are counted on each side for a total of 18 large squares.
Side 1-- 186 nucleated cells counted
Side 2-- 184 nucleated cells counted
total nucleated cells = 370
Using the standard hemocytometer formula shown on the right, what is the nucleated cell count per microliter (µL)?
A) 4.62 x 10^4
B) 2.06 x 10^4
C) 9.25 x 10^4
D) 1.03 x 10^4
2. The characteristic that distinguishes vancomycin-resistant Enterococcus faecium and Enterococcus faecalis from other Enterococcus species is their lack of motility.
Enterococci are all catalase-negative. Growth on bile esculin agar and in 6.5% salt broth are two characteristics that have commonly been used to identify Enterococcus to the genus level. A positive esculin in combination with a positive PYR reaction is another approach to presumptive identification.
Which one of these characteristics distinguishes vancomycin-resistant Enterococcus faecium and Enterococcus faecalis from other Enterococcus species?
A) Catalase negative
B) Non-motility
C) Growth on bile esculin agar
D) PYR positive reaction
3. The term universal precautions refers to:
A) mixing organic chemicals under a hood.
B) treating only CERTAIN specimens as infectious.
C) universal blood donors.
D) wellness clinic available to everyone.
4. Hematology
What is Coombs sera comprised of:
A) Complement
B) Indicator red cells
C) Anti-human globulins
D) Anti-red cell globulins
5. Which of these advancements in the clinical laboratory has led to concerns about patient privacy
A) automated analyzers
B) high test volumes
C) computers
D) staffing with several levels of lab professionals
Solutions:
| Question # 1 Answer: B | Question # 2 Answer: B | Question # 3 Answer: B | Question # 4 Answer: C | Question # 5 Answer: C |
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